Abstract: The paper presents the results of the impact of longterm different crop rotation, manure and mineral nitrogen fertilization on soil organic carbon, total nitrogen, available form phosphorus and potassium content and pH properties. This study was based on a three-factor experiment, carried out since 1979 on a loamy sand (Albic Luvisol), in which arable crops were grown in two 4years rotations: RotA (grain maize, winter wheat, spring barley and silage maize) and RotB (grain maize, winter wheat plus mustard, spring barley and grass-clover ley). After 36 years, the soil in RotB with an increased input of organic matter (mustard and grass-clover ley) accumulated significantly larger amounts of soil organic carbon. However, in the absence of liming, the soil in RotB, contrary to that in RotA, became more acidic and had less amounts of available form phosphorus and potassium. The beneficial effect use of manure every 4 years in doses 40 tha-1, without liming was a in a both crop rotation, especially in RotB. The results from this longterm fertilization experiment indicate that application of manure fertilizer alone is not sufficient to maintain levels of soil organic carbon and nutrients under conditions of conventional management without aboveground crop residues, such as straw or mustard that would be returned to the soil. Regular application of FYM combined with legumes increases soil organic carbon and soil nitrogen content, but does not prevent a pH decease. However, in this rotation its does not prevent a decrease in soil available forms potassium and phosphorus content either. The mineral nitrogen fertilization prevents a steady decrease in the soil organic carbon level in soil.