The study investigated the effects of the uses of cattle manure, compost, and rice husk biochar (RHB) as un-combined and co-applications, emphasizing their lasting effects over multiple cropping cycles of plant bioassay. This approach holds promise for enhancing agronomic efficiency and cost-effectiveness and decreasing reliance on synthetic fertilizers. The experiment employed a factorial arrangement with two factors: organic amendment types (none, cattle manure, compost) and the application rates of RHB (0%, 2%, 4% w w^-1). Corn was used as the plant indicator over three bioassay cycles in a tropical sandy soil, with a one-time organic amendment before the first cycle. Corn biomass, plant nutrient content, soil-plant nutrient interactions, and their interrelationships via the principal component analysis were determined. Each un-combined organic amendment, whether RHB, cattle manure, or compost, could enhance soil fertility and corn growth from the first cropping cycle and sustain these improvements into the second and third cycles. However, certain co-applications of the amendments in the first cropping cycle, specifically cattle manure with 4%RHB and compost with 2% and 4%RHB, initially hindered corn growth due to K antagonistic to Ca and Mg. These adverse effects were mitigated over time, leading to positive outcomes in the second and third cycles. The study conclusively demonstrated that integrating cattle manure and compost with RHB could enhance soil fertility and plant growth sustainably, offering an alternative to synthetic fertilizers.