The objective of the paper was to investigate the abundance of available forms of macroelements in the subsoil on land under intensive use, and to assess whether deeper soil layers can serve a function in the nutrition of crops. Samples were collected for layers: 0–25, 26–50, and 51–75 cm from 8 boreholes with a diameter of 20 cm, whereas samples were collected from opposite walls of two of them. The pH value of all collected samples and the content of available forms of macronutrients: mineral nitrogen, phosphorus, potassium, magnesium and calcium were examined. Then the mean content of nutrients was compared by each layer. The Mehlich-3 method was used for the extraction of P, K, Mg and Ca, then the content was determined by the atomic absorption spectrometry (AAS) method. The spectrophotometric measurement of mineral nitrogen was made using a Solar flow analyzer with a cadmium column. Nitrogen extraction was performed from dry soil using a 1% K₂SO₄ solution in a soil-solution ratio of 1:10. In the statistical analysis, the average contents of components and pH in individual layers were compared using Tukey's test (p=0.05). The obtained results show a trend for transport down the soil profile of phosphorus, calcium, and magnesium. The study evidences that the application of mineral fertilisers increases the abundance of nutrients in soils at the analysed depths, and crop rotation should involve deep-rooting crops that can efficiently use the subsoil as a source of nutrients. Proper arrangement of crop rotation with deep-rooting species can contribute to a reduction of leaching of nutrients. This should translate into more efficient use of fertilisers, and therefore less dispersal of nutrients in the environment, as well as increased economic benefits.